Device

Part:BBa_K1448013:Design

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-10-19)


In E coli mono-terpene production cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First, digest this part with SpeI.

Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.

Third, mix and ligate both samples.


Various monoterpenes can be produced in E. coli cells containing this device and monoterpene synthase genes without exogenous GPP.



Source

This is a composite part consisting of K314110, K314111, R0011, B0034 and K517005.

References